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将毛细血管纳入内皮细胞珠发芽试验
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay
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将毛细血管纳入内皮细胞珠发芽试验

7,829 Views

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06:21 min

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February 16, 2018

DOI:

10.3791/57309-v

DOI:
10.3791/57309-v

•

06:21 min
February 16, 2018

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15 Views
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1Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, 2Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill, 3Tıp Fakültesi,University of North Carolina at Chapel Hill

Özet

Automatically generated

本协议提出了一种新的体外微珠分析方法, 更恰当地模拟体内的过程, 并结合毛细血管。这种修改使珠粒化验更忠实地重述内皮细胞和壁画细胞之间的异型细胞相互作用, 对血管生成至关重要。

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PericytesEndothelial CellsAngiogenesisSprouting AssayHeterotypic Cellular InteractionsMicrocarrier BeadsCell CultureProliferationMigrationAnastomosis

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Cite this Article

Azam, S. H., Smith, M., Somasundaram, V., Pecot, C. V. Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay. J. Vis. Exp. (132), e57309, doi:10.3791/57309 (2018).

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