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人間の腸 Organoids 上皮関門の透過性のリアルタイム計測
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
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人間の腸 Organoids 上皮関門の透過性のリアルタイム計測

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08:04 min

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December 18, 2017

DOI:

10.3791/56960-v

DOI:
10.3791/56960-v

•

08:04 min
December 18, 2017

•

4 Views
, , , ,
1Department of Internal Medicine, Division of Gastroenterology,University of Michigan, 2Department of Cell and Developmental Biology,University of Michigan, 3Department of Internal Medicine, Division of Infectious Disease,University of Michigan

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このプロトコルは、蛍光顕微鏡を用いたヒトの腸オルガノイドのリアルタイム次の薬理学的治療における上皮バリア透過性の測定について説明し、細胞顕微鏡検査をライブします。

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Hill, D. R., Huang, S., Tsai, Y., Spence, J. R., Young, V. B. Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids. J. Vis. Exp. (130), e56960, doi:10.3791/56960 (2017).

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