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Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

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08:04 min

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December 18, 2017

DOI:

10.3791/56960-v

DOI:
10.3791/56960-v

•

08:04 min
December 18, 2017

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14 Views
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1Department of Internal Medicine, Division of Gastroenterology,University of Michigan, 2Department of Cell and Developmental Biology,University of Michigan, 3Department of Internal Medicine, Division of Infectious Disease,University of Michigan

Tags

Epithelial Barrier PermeabilityHuman Intestinal OrganoidsReal-time MeasurementFITC-dextranMicroinjectionGastroenterologyToxinsBacterial ProductsPharmacologic TreatmentsVisualizationQuantitative Measure

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Hill, D. R., Huang, S., Tsai, Y., Spence, J. R., Young, V. B. Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids. J. Vis. Exp. (130), e56960, doi:10.3791/56960 (2017).

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