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Biologia do Desenvolvimento
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Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

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08:04 min

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December 18, 2017

DOI:

10.3791/56960-v

DOI:
10.3791/56960-v

•

08:04 min
December 18, 2017

•

4 Views
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1Department of Internal Medicine, Division of Gastroenterology,University of Michigan, 2Department of Cell and Developmental Biology,University of Michigan, 3Department of Internal Medicine, Division of Infectious Disease,University of Michigan

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This protocol describes the measurement of epithelial barrier permeability in real-time following pharmacologic treatment in human intestinal organoids using fluorescent microscopy and live cell microscopy.

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Hill, D. R., Huang, S., Tsai, Y., Spence, J. R., Young, V. B. Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids. J. Vis. Exp. (130), e56960, doi:10.3791/56960 (2017).

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