Journal
/
发育生物学
/
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

13,770 Views

•

08:04 min

•

December 18, 2017

DOI:

10.3791/56960-v

DOI:
10.3791/56960-v

•

08:04 min
December 18, 2017

•

4 Views
, , , ,
1Department of Internal Medicine, Division of Gastroenterology,University of Michigan, 2Department of Cell and Developmental Biology,University of Michigan, 3Department of Internal Medicine, Division of Infectious Disease,University of Michigan

Summary

Automatically generated

This protocol describes the measurement of epithelial barrier permeability in real-time following pharmacologic treatment in human intestinal organoids using fluorescent microscopy and live cell microscopy.

Explore More Videos

Epithelial Barrier PermeabilityHuman Intestinal OrganoidsReal-time MeasurementFITC-dextranMicroinjectionGastroenterologyToxinsBacterial ProductsPharmacologic TreatmentsVisualizationQuantitative Measure

Read Article

Cite this Article

Hill, D. R., Huang, S., Tsai, Y., Spence, J. R., Young, V. B. Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids. J. Vis. Exp. (130), e56960, doi:10.3791/56960 (2017).

Download .ris file

Copy

Share Video

.

Copy