Dergi
/
Genetik
/
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
JoVE Journal
Genetik
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Genetik
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

10,317 Views

•

13:03 min

•

March 08, 2018

DOI:

10.3791/56837-v

DOI:
10.3791/56837-v

•

13:03 min
March 08, 2018

•

4 Views
, ,
1Donald Danforth Plant Science Center, 2Department of Biological Sciences,The University of Alabama

Özet

Automatically generated

This article demonstrates a detailed protocol for DNA isolation and high-throughput sequencing library construction from herbarium material including rescue of exceptionally poor-quality DNA.

Explore More Videos

DNA IsolationHigh-throughput SequencingHerbarium SpecimensPhylogenomic StudiesCTAB ExtractionRNase APhenol-chloroform PurificationLibrary Construction

Read Article

Cite this Article

Saeidi, S., McKain, M. R., Kellogg, E. A. Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens. J. Vis. Exp. (133), e56837, doi:10.3791/56837 (2018).

Download .ris file

Copy

Share Video

.

Copy