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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
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JoVE Journal Genetica
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

10,325 Views

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13:03 min

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March 08, 2018

DOI:

10.3791/56837-v

DOI:
10.3791/56837-v

•

13:03 min
March 08, 2018

•

4 Views
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1Donald Danforth Plant Science Center, 2Department of Biological Sciences,The University of Alabama

Summary

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This article demonstrates a detailed protocol for DNA isolation and high-throughput sequencing library construction from herbarium material including rescue of exceptionally poor-quality DNA.

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DNA IsolationHigh-throughput SequencingHerbarium SpecimensPhylogenomic StudiesCTAB ExtractionRNase APhenol-chloroform PurificationLibrary Construction

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Cite this Article

Saeidi, S., McKain, M. R., Kellogg, E. A. Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens. J. Vis. Exp. (133), e56837, doi:10.3791/56837 (2018).

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