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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
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JoVE 杂志 遗传学
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

DOI:
10.3791/56837-v

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13:03 min

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March 08, 2018

•

, ,
1Donald Danforth Plant Science Center, 2Department of Biological Sciences,The University of Alabama

Chapters

  • 00:04Title
  • 00:58DNA Extraction
  • 04:39DNA Shearing
  • 05:28Bead Clean-up
  • 07:06Library Preparation
  • 07:40Adaptor Ligation
  • 08:46PCR Amplification
  • 09:12Size Selection for Desired Library Size and Quality Control
  • 11:04Results: Isolation and High-throughput Sequencing of Poor Quality DNA Isolated from Various Herbarium Species
  • 12:33Conclusion

Summary

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This article demonstrates a detailed protocol for DNA isolation and high-throughput sequencing library construction from herbarium material including rescue of exceptionally poor-quality DNA.

Tags

DNA IsolationHigh-throughput SequencingHerbarium SpecimensPhylogenomic StudiesCTAB ExtractionRNase APhenol-chloroform PurificationLibrary Construction

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