Dergi
/
Genetik
/
提高吞吐量的轰击大麦糊粉层层中基因表达的测定
JoVE Journal
Genetik
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Genetik
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Please note that all translations are automatically generated. Click here for the English version.

提高吞吐量的轰击大麦糊粉层层中基因表达的测定

DOI:
10.3791/56728-v

•

10:29 min

•

March 30, 2018

•

, , , ,
1Biyoloji Bölümü,Colby College

Bölümler

  • 00:05Başlık
  • 00:44Preparation of Barley Grains and Plasmid DNA for Bombardment
  • 02:42Particle Bombardment of Embryoless Barley Grains
  • 04:42Extraction of Soluble Protein from the Bombarded Grains
  • 06:24Measurement of GUS Activity from Grain Extracts
  • 08:15Results: Measurement of Gene Expression in Barley Aleurone Cells after Particle Bombardment
  • 09:50Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

提出了一种改进的协议, 用于测量粒子轰击后大麦糊粉层细胞中的瞬时基因表达。采用全自动谷物研磨与96井板酶检测相结合, 为该过程提供了较高的吞吐量。

Etiketler

Gene ExpressionBarley AleuroneParticle BombardmentReporter ConstructsAbscisic AcidHigh-throughputSeed Coat PeelingVermiculiteUBI LuciferaseGUS ReporterEffector PlasmidMicrocarriersParticle Delivery SystemRupture Disk

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

在人原代细胞培养生物钟基因表达及荷尔蒙分泌的并行测量

肠易激综合征的循环miRNA的扰动检测使用复用高通量基因表达平台

单细胞基因表达谱使用流式细胞仪和qPCR内部标准

通过两个互补细胞同步方案研究细胞周期调控的基因表达

斑马鱼 RNASeq-based 基因表达数据的样品制备与分析

量化的信息编码的基因表达水平在寿命调制下线虫的宽范围饮食限制

无基因克隆的基因破坏的变形链球菌菌株的生成

细胞间相互作用中基因表达模式的控制与分析的 Optogenetic 方法

一种基于昆虫蜕皮激素受体的奇异基因开关, 用于对转基因进行有鲁棒性、可逆性和可忽略的泄漏的刻意表达

从土壤、根际和根 Endosphere 提取细菌群落数据的根菌群探析

Read Article