需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
6,355 Views
•
10:29 min
•
March 30, 2018
DOI:
Summary
Automatically generated
An improved protocol is presented for the measurement of transient gene expression from reporter constructs in barley aleurone cells after particle bombardment. The combination of automated grain grinding with 96-well plate enzyme assays provides high throughput for the procedure.
Read Article
Cite this Article
Uwase, G., Enrico, T. P., Chelimo, D. S., Keyser, B. R., Johnson, R. R. Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput. J. Vis. Exp. (133), e56728, doi:10.3791/56728 (2018).
Copy