Rivista
/
Genetica
/
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

DOI:
10.3791/56728-v

•

10:29 min

•

March 30, 2018

•

, , , ,
1Department of Biology,Colby College

Capitoli

  • 00:05Titolo
  • 00:44Preparation of Barley Grains and Plasmid DNA for Bombardment
  • 02:42Particle Bombardment of Embryoless Barley Grains
  • 04:42Extraction of Soluble Protein from the Bombarded Grains
  • 06:24Measurement of GUS Activity from Grain Extracts
  • 08:15Results: Measurement of Gene Expression in Barley Aleurone Cells after Particle Bombardment
  • 09:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

An improved protocol is presented for the measurement of transient gene expression from reporter constructs in barley aleurone cells after particle bombardment. The combination of automated grain grinding with 96-well plate enzyme assays provides high throughput for the procedure.

Tags

Gene ExpressionBarley AleuroneParticle BombardmentReporter ConstructsAbscisic AcidHigh-throughputSeed Coat PeelingVermiculiteUBI LuciferaseGUS ReporterEffector PlasmidMicrocarriersParticle Delivery SystemRupture Disk

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Read Article