/
/
Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis
8,286 Views
•
07:38 min
•
November 03, 2017
DOI:
Özet
Automatically generated
This protocol describes using cultured Aorta-Gonad-Mesonephros for expression analyses, colony-forming units in the culture and spleen, and long-term reconstitution to determine the effect of regulatory factors and signaling pathways on hematopoietic stem cell development. This has been demonstrated as an effective system for studying hematopoietic stem cell biology and function.
Read Article
Cite this Article
Lv, J., Liu, F. Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis. J. Vis. Exp. (129), e56557, doi:10.3791/56557 (2017).
Copy