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Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis
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Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis
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November 03, 2017
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This protocol describes using cultured Aorta-Gonad-Mesonephros for expression analyses, colony-forming units in the culture and spleen, and long-term reconstitution to determine the effect of regulatory factors and signaling pathways on hematopoietic stem cell development. This has been demonstrated as an effective system for studying hematopoietic stem cell biology and function.
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Lv, J., Liu, F. Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis. J. Vis. Exp. (129), e56557, doi:10.3791/56557 (2017).
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