Journal
/
发育生物学
/
Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

DOI:
10.3791/56557-v

•

07:38 min

•

November 03, 2017

•

,
1State Key Laboratory of Membrane Biology, Institute of Zoology,Chinese Academy of Sciences, 2University of Chinese Academy of Sciences

Chapters

  • 00:05Title
  • 01:06Aorta-gonad-mesonephros (AGM) Explant Culture and Colony Forming Units CFU) Analysis |
  • 04:24In Vivo AGM Transplantation
  • 06:08Results: Representative Effects of Fluoxetine on Hematopoietic Stem Cell (HSC) Development
  • 07:08Conclusion

Summary

自动翻译

自动翻译

This protocol describes using cultured Aorta-Gonad-Mesonephros for expression analyses, colony-forming units in the culture and spleen, and long-term reconstitution to determine the effect of regulatory factors and signaling pathways on hematopoietic stem cell development. This has been demonstrated as an effective system for studying hematopoietic stem cell biology and function.

Tags

Keywords: Aorta-gonad-mesonephrosExplant CultureHematopoiesisHematopoietic Stem CellsHSC DevelopmentEmbryonicDissectionCollagenaseCell CultureM3434 Medium

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/56557?article_type=v

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System
check_url/cn/56557?article_type=v

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
check_url/cn/56557?article_type=v

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
check_url/cn/56557?article_type=v

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
check_url/cn/56557?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
check_url/cn/56557?article_type=v

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition
check_url/cn/56557?article_type=v

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
check_url/cn/56557?article_type=v

A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish
check_url/cn/56557?article_type=v

Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells
check_url/cn/56557?article_type=v

An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila

Read Article