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Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

10,444 Views

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10:33 min

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October 25, 2017

DOI:

10.3791/56514-v

DOI:
10.3791/56514-v

•

10:33 min
October 25, 2017

•

12 Views
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1Institute for Medical Microbiology, Immunology and Hygiene,University of Cologne, 2Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne

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We describe here a simple and quick method for the isolation of Salmonella typhimurium-containing phagosomes from macrophages by coating the bacteria with biotin and streptavidin.

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Salmonella TyphimuriumPhagosomesMacrophagesIsolationİmmünolojiAntigen ProcessingIntracellular PathogensBiotinStreptavidin-conjugated Magnetic Beads

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Gutiérrez, S., Wolke, M., Plum, G., Robinson, N. Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages. J. Vis. Exp. (128), e56514, doi:10.3791/56514 (2017).

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