Journal
/
Immunologie und Infektion
/
Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

10,444 Views

•

10:33 min

•

October 25, 2017

DOI:

10.3791/56514-v

DOI:
10.3791/56514-v

•

10:33 min
October 25, 2017

•

12 Views
, , ,
1Institute for Medical Microbiology, Immunology and Hygiene,University of Cologne, 2Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne

Summary

Automatically generated

We describe here a simple and quick method for the isolation of Salmonella typhimurium-containing phagosomes from macrophages by coating the bacteria with biotin and streptavidin.

Explore More Videos

Salmonella TyphimuriumPhagosomesMacrophagesIsolationImmunologyAntigen ProcessingIntracellular PathogensBiotinStreptavidin-conjugated Magnetic Beads

Read Article

Cite this Article

Gutiérrez, S., Wolke, M., Plum, G., Robinson, N. Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages. J. Vis. Exp. (128), e56514, doi:10.3791/56514 (2017).

Download .ris file

Copy

Share Video

.

Copy