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流感血凝素特异抗体诱导的 Fc 介导效应函数的一种评估方法
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
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流感血凝素特异抗体诱导的 Fc 介导效应函数的一种评估方法

DOI:
10.3791/56256-v

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04:47 min

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February 23, 2018

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, , ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3Department of Infectious Disease,J. Craig Venter Institute

Bölümler

  • 00:05Başlık
  • 00:42Influenza Virus Hemagglutinin Expression
  • 02:18Antibody-dependent Cell-mediated Cytotoxicity (ADCC) Analysis
  • 04:01Results: Representative ADCC Analysis
  • 04:24Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

我们描述了一种方法来衡量 Fc 介导的效应函数的活化作用的抗体, 针对流感病毒血凝素。这种检测也可以适应评估单克隆抗体或多克隆血清靶向其他病毒表面糖蛋白, 以诱导 Fc 介导的免疫能力。

Etiketler

Fc-mediated Effector FunctionsInfluenza HemagglutininAntibodyADCCCell-mediated CytotoxicityTransfectionInfectionA549 Cells293 CellsVirusİmmünolojiVaccinology

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