Dergi
/
İmmünoloji ve Enfeksiyon
/
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

7,557 Views

•

04:47 min

•

February 23, 2018

DOI:

10.3791/56256-v

DOI:
10.3791/56256-v

•

04:47 min
February 23, 2018

•

1 Views
, , ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3Department of Infectious Disease,J. Craig Venter Institute

Özet

Automatically generated

We describe a method to measure the activation of Fc-mediated effector functions by antibodies that target the influenza virus hemagglutinin. This assay can also be adapted to assess the ability of monoclonal antibodies or polyclonal sera targeting other viral surface glycoproteins to induce Fc-mediated immunity.

Explore More Videos

Fc-mediated Effector FunctionsInfluenza HemagglutininAntibodyADCCCell-mediated CytotoxicityTransfectionInfectionA549 Cells293 CellsVirusİmmünolojiVaccinology

Read Article

Cite this Article

Bailey, M. J., Broecker, F., Leon, P. E., Tan, G. S. A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies. J. Vis. Exp. (132), e56256, doi:10.3791/56256 (2018).

Download .ris file

Copy

Share Video

.

Copy