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A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
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JoVE Journal 免疫学と感染
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

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04:47 min

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February 23, 2018

DOI:

10.3791/56256-v

DOI:
10.3791/56256-v

•

04:47 min
February 23, 2018

•

1 Views
, , ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3Department of Infectious Disease,J. Craig Venter Institute

概要

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We describe a method to measure the activation of Fc-mediated effector functions by antibodies that target the influenza virus hemagglutinin. This assay can also be adapted to assess the ability of monoclonal antibodies or polyclonal sera targeting other viral surface glycoproteins to induce Fc-mediated immunity.

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Cite this Article

Bailey, M. J., Broecker, F., Leon, P. E., Tan, G. S. A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies. J. Vis. Exp. (132), e56256, doi:10.3791/56256 (2018).

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