Dergi
/
Genetik
/
Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
JoVE Journal
Genetik
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Genetik
Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

8,131 Views

•

09:45 min

•

August 10, 2017

DOI:

10.3791/56226-v

DOI:
10.3791/56226-v

•

09:45 min
August 10, 2017

•

4 Views
, , ,
1Department of Molecular Biology,Rowan School of Osteopathic Medicine, 2Department of Biological Sciences,Rowan University

Özet

Automatically generated

Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular response.

Explore More Videos

MRNA LevelsS. CerevisiaeHypoxic ResponseGene ExpressionTime CourseLiquid CultureNitrogen TankYPD MediaPre-cultureRNA-seq AnalysisCellular ResponseSignaling PathwaysGene Regulation

Read Article

Cite this Article

Willis, S. D., Hossian, A. K. M. N., Evans, N., Hickman, M. J. Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response. J. Vis. Exp. (126), e56226, doi:10.3791/56226 (2017).

Download .ris file

Copy

Share Video

.

Copy