Measuring mRNA Levels Over Time During the Yeast <em>S. cerevisiae</em> Hypoxic Response

Stephen D. Willis; A. K. M. Nawshad Hossian; Nathan Evans; Mark J. Hickman

doi:10.3791/56226

'Dergi'

/

Genetik

/

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

JoVE Journal
Genetik

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JoVE Journal Genetik

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

DOI:

10.3791/56226-v

•

09:45 min

•

August 10, 2017

•

Stephen D. Willis, A. K. M. Nawshad Hossian, Nathan Evans, Mark J. Hickman

¹Department of Molecular Biology,Rowan School of Osteopathic Medicine, ²Department of Biological Sciences,Rowan University

'Bölümler'

00:05'Başlık'
00:53Preparation Prior to Inducing Hypoxia
03:52Inducing Hypoxia and Collecting Samples
07:37Results: Gene Expression Changes During the Hypoxic Response of S. cerevisiae
09:12Conclusion

Özet

'Otomatik Çeviri'

Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular response.

'Etiketler'

MRNA Levels S. Cerevisiae Hypoxic Response Gene Expression Time Course Liquid Culture Nitrogen Tank YPD Media Pre-culture RNA-seq Analysis Cellular Response Signaling Pathways Gene Regulation

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