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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
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Genética
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JoVE Journal Genética
Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

8,131 Views

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09:45 min

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August 10, 2017

DOI:

10.3791/56226-v

DOI:
10.3791/56226-v

•

09:45 min
August 10, 2017

•

4 Views
, , ,
1Department of Molecular Biology,Rowan School of Osteopathic Medicine, 2Department of Biological Sciences,Rowan University

Summary

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Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular response.

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Willis, S. D., Hossian, A. K. M. N., Evans, N., Hickman, M. J. Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response. J. Vis. Exp. (126), e56226, doi:10.3791/56226 (2017).

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