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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

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JoVE 杂志遗传学

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

DOI:

10.3791/56226-v

•

09:45 min

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August 10, 2017

•

Stephen D. Willis, A. K. M. Nawshad Hossian, Nathan Evans, Mark J. Hickman

¹Department of Molecular Biology,Rowan School of Osteopathic Medicine, ²Department of Biological Sciences,Rowan University

Chapters

00:05Title
00:53Preparation Prior to Inducing Hypoxia
03:52Inducing Hypoxia and Collecting Samples
07:37Results: Gene Expression Changes During the Hypoxic Response of S. cerevisiae
09:12Conclusion

Summary

自动翻译

Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular response.

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