Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
9,686 Views
•
13:21 min
•
June 16, 2017
DOI:
Özet
Automatically generated
This work describes an optimized methyl-CpG-binding domain (MBD) sequencing protocol and a computational pipeline to identify differentially methylated CpG-rich regions in chronic lymphocytic leukemia (CLL) patients.
Explore More Videos
DNA MethylationMethyl-CpG-binding DomainCapture-based MethodChronic Lymphocytic LeukemiaNext-generation SequencingGlobal Methylation ProfilingDifferentially Methylated CpG-rich RegionsCLL-specific Signature GenesDisease PathogenesisPrognosisGenomic DNA ExtractionSonicationMagnetic Streptavidin BeadsMBD-biotin Protein
Read Article
Cite this Article
Subhash, S., Kanduri, M. Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients. J. Vis. Exp. (124), e55773, doi:10.3791/55773 (2017).
Copy