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Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
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JoVE Journal Genética
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

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13:21 min

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June 16, 2017

DOI:

10.3791/55773-v

DOI:
10.3791/55773-v

•

13:21 min
June 16, 2017

•

4 Views
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1Department of Medical Genetics, Institute of Biomedicine,Gothenburg University, 2Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine,Gothenburg University

Summary

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This work describes an optimized methyl-CpG-binding domain (MBD) sequencing protocol and a computational pipeline to identify differentially methylated CpG-rich regions in chronic lymphocytic leukemia (CLL) patients.

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DNA MethylationMethyl-CpG-binding DomainCapture-based MethodChronic Lymphocytic LeukemiaNext-generation SequencingGlobal Methylation ProfilingDifferentially Methylated CpG-rich RegionsCLL-specific Signature GenesDisease PathogenesisPrognosisGenomic DNA ExtractionSonicationMagnetic Streptavidin BeadsMBD-biotin Protein

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Subhash, S., Kanduri, M. Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients. J. Vis. Exp. (124), e55773, doi:10.3791/55773 (2017).

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