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リン酸化部位決定のためのオリゴペプチド競合アッセイ

JoVE Journal
Biyokimya

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JoVE Journal Biyokimya

Oligopeptide Competition Assay for Phosphorylation Site Determination

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リン酸化部位決定のためのオリゴペプチド競合アッセイ

8,289 Views

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09:16 min

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May 18, 2017

DOI:

10.3791/55708-v

DOI:

10.3791/55708-v

•

09:16 min

May 18, 2017

•

8 Views

Min Sung Joo¹, Ja Hyun Koo¹, Sol-Bi Shin², Hyungshin Yim², Sang Geon Kim¹

¹College of Pharmacy and Research Institute of Pharmaceutical Sciences,Seoul National University, ²College of Pharmacy and Institute of Pharmaceutical Science and Technology,Hanyang University

Özet

ペプチド競合アッセイは、種々の分子および免疫学的実験において広く使用されている。本稿では、in vitroオリゴペプチド競合キナーゼアッセイの詳細な方法と、特定のリン酸化部位を見出すのに有用な関連する検証手順について説明します。

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