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精密切割的小鼠肺切片可视化现场肺树突状细胞
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JoVE Journal İmmünoloji ve Enfeksiyon
Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
Please note that all translations are automatically generated. Click here for the English version.

精密切割的小鼠肺切片可视化现场肺树突状细胞

16,353 Views

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09:33 min

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April 05, 2017

DOI:

10.3791/55465-v

DOI:
10.3791/55465-v

•

09:33 min
April 05, 2017

•

24 Views
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1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research,National Institute of Environmental Health Sciences, NIH

Özet

Automatically generated

我们描述了用于产生精密切割肺片（PCLS）和免疫它们以显现各种免疫细胞类型的定位在肺中的方法。我们的协议可以扩展在各种条件下可视化的位置和许多不同细胞类型的功能。

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Cite this Article

Lyons-Cohen, M. R., Thomas, S. Y., Cook, D. N., Nakano, H. Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells. J. Vis. Exp. (122), e55465, doi:10.3791/55465 (2017).

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