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Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry
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JoVE Journal Nörobilim
Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

10,565 Views

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08:36 min

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March 23, 2017

DOI:

10.3791/55254-v

DOI:
10.3791/55254-v

•

08:36 min
March 23, 2017

•

7 Views
, , , ,
1Anatomi ve Hücre Biyolojisi Anabilim Dalı,The University of Western Ontario, 2Department of Chemistry and Biochemistry,The University of Western Ontario

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A protocol for the sublimation of DAN matrix onto rat brain tissue for the detection of gangliosides using MALDI Imaging Mass Spectrometry is presented.

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GangliosidesMALDI Imaging Mass SpectrometrySublimationDAN MatrixCryostat SectioningLipid DetectionNeurobiology

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Caughlin, S., Park, D. H., Yeung, K. K., Cechetto, D. F., Whitehead, S. N. Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry. J. Vis. Exp. (121), e55254, doi:10.3791/55254 (2017).

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