A protocol for the sublimation of DAN matrix onto rat brain tissue for the detection of gangliosides using MALDI Imaging Mass Spectrometry is presented.
Caughlin, S., Park, D. H., Yeung, K. K., Cechetto, D. F., Whitehead, S. N. Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry. J. Vis. Exp. (121), e55254, doi:10.3791/55254 (2017).