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Neuroscience

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JoVE Journal Neuroscience
Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry
 

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

Article DOI: 10.3791/55254-v • 08:36 min • March 23rd, 2017
March 23rd, 2017 •
1, 1, 2, 1, 1
1Department of Anatomy and Cell Biology, The University of Western Ontario, 2Department of Chemistry and Biochemistry, The University of Western Ontario

Kapitel

Summary
Automatic Translation

A protocol for the sublimation of DAN matrix onto rat brain tissue for the detection of gangliosides using MALDI Imaging Mass Spectrometry is presented.

Tags

Sublimation DAN Matrix Detection Visualization Gangliosides Rat Brain Tissue MALDI Imaging Mass Spectrometry Sample Preparation Neurobiology Anatomical Distribution Function Brain Pathology High-quality Imaging Lipid Species Cryostat Cutting Thickness Cryostat Anti-roll Bar Slicing Platform Conductive Slides Metal Plates
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