Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Adam Z. Albitar; Wanlong Ma; Maher Albitar

doi:10.3791/55130

'Dergi'

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Genetik

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野生类型阻止PCR结合直接测序作为低频体细胞突变的检测的高灵敏度的方法

JoVE Journal
Genetik

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JoVE Journal Genetik

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Please note that all translations are automatically generated. Click here for the English version.

野生类型阻止PCR结合直接测序作为低频体细胞突变的检测的高灵敏度的方法

DOI:

10.3791/55130-v

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10:41 min

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March 29, 2017

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Adam Z. Albitar, Wanlong Ma, Maher Albitar

¹NeoGenomics Laboratories

'Bölümler'

00:05'Başlık'
00:43Wild-Type Blocking PCR: Oligonucleotide Design
03:54Purification of PCR Product by Magnetic Beads
05:53Purification of the Sequencing Products
08:17Identification of MYD88 Mutations in Human Samples
10:03Conclusion

Özet

'Otomatik Çeviri'

Wild-type blocking PCR followed by direct sequencing offers a highly sensitive method of detection for low frequency somatic mutations in a variety of sample types.

'Etiketler'

Keyword Extraction:Wild-type Blocking PCR Somatic Mutations Myd88 Gene Bone Marrow Samples Exon Five L265 Hotspot M13 Sequence Blocking Oligonucleotide Wild-type Template Melting Temperature Extension Temperature Secondary Structure Self-dimerization

野生类型阻止PCR结合直接测序作为低频体细胞突变的检测的高灵敏度的方法

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