Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
9,321 Views
•
10:08 min
•
March 05, 2017
DOI:
Özet
Automatically generated
Many developmentally important genes have cell- or tissue-specific expression patterns. This paper describes LM RNA-seq experiments to identify genes that are differentially expressed at the maize leaf blade-sheath boundary and in lg1-R mutants compared to wild-type. The experimental considerations discussed here apply to transcriptomic analyses of other developmental phenomena.
Read Article
Cite this Article
Johnston, R. M., Sylvester, A. W., Scanlon, M. J. Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development. J. Vis. Exp. (121), e55004, doi:10.3791/55004 (2017).
Copy