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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
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March 05, 2017
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Many developmentally important genes have cell- or tissue-specific expression patterns. This paper describes LM RNA-seq experiments to identify genes that are differentially expressed at the maize leaf blade-sheath boundary and in lg1-R mutants compared to wild-type. The experimental considerations discussed here apply to transcriptomic analyses of other developmental phenomena.
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Johnston, R. M., Sylvester, A. W., Scanlon, M. J. Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development. J. Vis. Exp. (121), e55004, doi:10.3791/55004 (2017).
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