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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
Kapitel
- 00:05Titel
- 00:45Tissue Fixation and Processing
- 03:03Sectioning and Slide Preparation
- 04:57Microdissection of Blade, Ligule, and Sheath Samples from Plastochron 7 Leaf Primordia
- 07:48Results: Laser Microdissection RNA Sequencing Identifies Differentially Expressed Genes in Maize Leaf
- 09:38Conclusion
Many developmentally important genes have cell- or tissue-specific expression patterns. This paper describes LM RNA-seq experiments to identify genes that are differentially expressed at the maize leaf blade-sheath boundary and in lg1-R mutants compared to wild-type. The experimental considerations discussed here apply to transcriptomic analyses of other developmental phenomena.
