Dergi
/
Genetik
/
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
JoVE Journal
Genetik
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Genetik
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

10,186 Views

•

09:57 min

•

December 28, 2016

DOI:

10.3791/54976-v

DOI:
10.3791/54976-v

•

09:57 min
December 28, 2016

•

23 Views
, ,
1Entomology Department,University of Maryland, 2Program in Molecular and Cell Biology,University of Maryland

Özet

Automatically generated

Here, we present protocols for rearing an intermediate-germ beetle, Dermestes maculatus (D. maculatus) in the lab. We also share protocols for embryonic and parental RNAi and methods for analyzing embryonic phenotypes to study gene function in this species.

Explore More Videos

Dermestes MaculatusHide BeetleRearingDouble-stranded RNAGene KnockdownInsect Model SystemRNA InterferenceGelişim BiyolojisiEvolutionary BiologyInsect Pest Control

Read Article

Cite this Article

Xiang, J., Reding, K., Pick, L. Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus. J. Vis. Exp. (118), e54976, doi:10.3791/54976 (2016).

Download .ris file

Copy

Share Video

.

Copy