Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, <em>Dermestes maculatus</em>

Jie Xiang; Katie Reding; Leslie Pick

doi:10.3791/54976

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遺伝学

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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

10,186 Views

•

09:57 min

•

December 28, 2016

DOI:

10.3791/54976-v

DOI:

10.3791/54976-v

•

09:57 min

December 28, 2016

•

23 Views

Jie Xiang^1,2, Katie Reding¹, Leslie Pick^1,2

¹Entomology Department,University of Maryland, ²Program in Molecular and Cell Biology,University of Maryland

概要

Here, we present protocols for rearing an intermediate-germ beetle, Dermestes maculatus (D. maculatus) in the lab. We also share protocols for embryonic and parental RNAi and methods for analyzing embryonic phenotypes to study gene function in this species.

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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

概要

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