Journal
/
遗传学
/
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
JoVE 杂志
遗传学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 遗传学
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

DOI:
10.3791/54976-v

•

09:57 min

•

December 28, 2016

•

, ,
1Entomology Department,University of Maryland, 2Program in Molecular and Cell Biology,University of Maryland

Chapters

  • 00:05Title
  • 01:00D. maculatus Rearing
  • 01:37D. maculatus Embryo Collection
  • 02:58Embryonic RNAi (eRNAi)
  • 05:14Parental RNAi (pRNAi)
  • 07:04Results: Roles of Dmac-prd in Body Segment Formation Were Demonstrated Using pRNAi
  • 08:38Conclusion

Summary

自动翻译

自动翻译

Here, we present protocols for rearing an intermediate-germ beetle, Dermestes maculatus (D. maculatus) in the lab. We also share protocols for embryonic and parental RNAi and methods for analyzing embryonic phenotypes to study gene function in this species.

Tags

Dermestes MaculatusHide BeetleRearingDouble-stranded RNAGene KnockdownInsect Model SystemRNA Interference发育生物学Evolutionary BiologyInsect Pest Control

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Production and Purification of Baculovirus for Gene Therapy Application

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Read Article