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从HIV-1感染细胞的外来体基于SILAC蛋白质组学表征
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JoVE Journal İmmünoloji ve Enfeksiyon
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
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从HIV-1感染细胞的外来体基于SILAC蛋白质组学表征

10,485 Views

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10:24 min

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March 03, 2017

DOI:

10.3791/54799-v

DOI:
10.3791/54799-v

•

10:24 min
March 03, 2017

•

2 Views
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1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories,Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine,Warren Alpert Medical School, Brown University

Özet

Automatically generated

在这里，我们描述了使用通过在细胞培养物（SILAC）氨基酸稳定同位素标记的技术来分析HIV-1感染的主机外来体蛋白质组的作用的定量蛋白质组学方法。该协议可以很容易地适应于不同的压力或感染的条件下的细胞。

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SILACProteomic CharacterizationExosomesHIV-1 InfectionCell CultureLabelingUltracentrifugationMass Spectrometry

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Cite this Article

Cheruiyot, C., Pataki, Z., Williams, R., Ramratnam, B., Li, M. SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells. J. Vis. Exp. (121), e54799, doi:10.3791/54799 (2017).

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