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SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

10,485 Views

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10:24 min

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March 03, 2017

DOI:

10.3791/54799-v

DOI:
10.3791/54799-v

•

10:24 min
March 03, 2017

•

2 Views
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1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories,Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine,Warren Alpert Medical School, Brown University

Summary

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Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.

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SILACProteomic CharacterizationExosomesHIV-1 InfectionCell CultureLabelingUltracentrifugationMass Spectrometry

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Cheruiyot, C., Pataki, Z., Williams, R., Ramratnam, B., Li, M. SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells. J. Vis. Exp. (121), e54799, doi:10.3791/54799 (2017).

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