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SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
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SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
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March 03, 2017
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Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.
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Cheruiyot, C., Pataki, Z., Williams, R., Ramratnam, B., Li, M. SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells. J. Vis. Exp. (121), e54799, doi:10.3791/54799 (2017).
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