Journal
/
Imunologia e Infecção
/
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

10,485 Views

•

10:24 min

•

March 03, 2017

DOI:

10.3791/54799-v

DOI:
10.3791/54799-v

•

10:24 min
March 03, 2017

•

2 Views
, , , ,
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories,Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine,Warren Alpert Medical School, Brown University

Summary

Automatically generated

Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.

Explore More Videos

SILACProteomic CharacterizationExosomesHIV-1 InfectionCell CultureLabelingUltracentrifugationMass Spectrometry

Read Article

Cite this Article

Cheruiyot, C., Pataki, Z., Williams, R., Ramratnam, B., Li, M. SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells. J. Vis. Exp. (121), e54799, doi:10.3791/54799 (2017).

Download .ris file

Copy

Share Video

.

Copy