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실험자가 면역 뇌척수염 연구 간단하고 효율적인 생산 및 마우스 미엘린 희소 돌기 아교 세포 당 단백질의 정제
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies
Please note that all translations are automatically generated. Click here for the English version.

실험자가 면역 뇌척수염 연구 간단하고 효율적인 생산 및 마우스 미엘린 희소 돌기 아교 세포 당 단백질의 정제

10,749 Views

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10:47 min

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October 27, 2016

DOI:

10.3791/54727-v

DOI:
10.3791/54727-v

•

10:47 min
October 27, 2016

•

3 Views
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1Department of Microbiology and Immunology,Western University

Özet

Automatically generated

We describe a simple protocol using only basic lab equipment to generate and purify large quantities of a fusion protein that contains mouse Myelin Oligodendrocyte Glycoprotein. This protein can be used to induce experimental autoimmune encephalomyelitis driven by both T and B cells.

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Jain, R. W., Dang, A. K., Kerfoot, S. M. Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies. J. Vis. Exp. (116), e54727, doi:10.3791/54727 (2016).

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