Dergi
/
Biyokimya
/
Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

DOI:
10.3791/54640-v

•

10:44 min

•

October 21, 2016

•

, ,
1Departamento de Química de Biomacromoléculas, Instituto de Química,Universidad Nacional Autónoma de México, 2Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología,Universidad Nacional Autónoma de México

Bölümler

  • 00:05Başlık
  • 00:36SBDS-FlAsH Tag Protein Expression and Purification
  • 04:10Elongation Factor Like-1 (EFL1) Protein Expression and Purification
  • 07:07Labeling of SBDS-FlAsH with the FlAsH Fluorescent Dye 4′,5′-Bis(1,3,2 dithioarsolan-2-yl) Fluorescein and Fluorescence Anisotropy Experiments
  • 08:50Results: Evaluation of Protein-protein Interactions Using Fluorescence Anisotropy
  • 09:55Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Protein interactions are at the heart of a cell's function. Calorimetric and spectroscopic techniques are commonly used to characterize them. Here we describe fluorescence anisotropy as a tool to study the interaction between the protein mutated in the Shwachman-Diamond Syndrome (SBDS) and the Elongation factor-like 1 GTPase (EFL1).

Etiketler

Fluorescence AnisotropyProtein-protein InteractionsProtein BiochemistryProtein BiophysicsSBDS-FlAsH ProteinProtein PurificationNickel Affinity ColumnSulfopropyl Cation Exchanger ColumnProtein Concentration

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Read Article