Journal
/
Bioquímica
/
Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

29,816 Views

•

10:44 min

•

October 21, 2016

DOI:

10.3791/54640-v

DOI:
10.3791/54640-v

•

10:44 min
October 21, 2016

•

14 Views
, ,
1Departamento de Química de Biomacromoléculas, Instituto de Química,Universidad Nacional Autónoma de México, 2Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología,Universidad Nacional Autónoma de México

Summary

Automatically generated

Protein interactions are at the heart of a cell's function. Calorimetric and spectroscopic techniques are commonly used to characterize them. Here we describe fluorescence anisotropy as a tool to study the interaction between the protein mutated in the Shwachman-Diamond Syndrome (SBDS) and the Elongation factor-like 1 GTPase (EFL1).

Explore More Videos

Fluorescence AnisotropyProtein-protein InteractionsProtein BiochemistryProtein BiophysicsSBDS-FlAsH ProteinProtein PurificationNickel Affinity ColumnSulfopropyl Cation Exchanger ColumnProtein Concentration

Read Article

Cite this Article

Gijsbers, A., Nishigaki, T., Sánchez-Puig, N. Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions. J. Vis. Exp. (116), e54640, doi:10.3791/54640 (2016).

Download .ris file

Copy

Share Video

.

Copy