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Technique to Target Microinjection to the Developing Xenopus Kidney
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Technique to Target Microinjection to the Developing Xenopus Kidney

Technique to Target Microinjection to the Developing Xenopus Kidney

DOI:
10.3791/53799-v

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11:29 min

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May 03, 2016

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, ,
1Department of Pediatrics, Pediatric Research Center,University of Texas McGovern Medical School, 2Program in Genes & Development,University of Texas Graduate School of Biomedical Sciences, 3Program in Cell & Regulatory Biology,University of Texas Graduate School of Biomedical Sciences, 4Genetik Bölümü,University of Texas MD Anderson Cancer Center

'Bölümler'

  • 00:05'Başlık'
  • 00:44Preparation of Embryos
  • 03:32Preparation of Injection Solutions and Microinjection of Embryos
  • 08:22Fixation and Immunostaining of Embryos
  • 09:42Results: Examples of Embryos Targeted at the 8-cell Stage
  • 10:56Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.

'Etiketler'

MicroinjectionXenopus KidneyGelişim BiyolojisiTestis IsolationEgg CollectionFertilizationDe-jellingTemperature ControlMembrane-bound Red Fluorescent Protein MRNA

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