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Technique to Target Microinjection to the Developing Xenopus Kidney
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JoVE Revista Biología del desarrollo
Technique to Target Microinjection to the Developing Xenopus Kidney

Technique to Target Microinjection to the Developing Xenopus Kidney

DOI:
10.3791/53799-v

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11:29 min

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May 03, 2016

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, ,
1Department of Pediatrics, Pediatric Research Center,University of Texas McGovern Medical School, 2Program in Genes & Development,University of Texas Graduate School of Biomedical Sciences, 3Program in Cell & Regulatory Biology,University of Texas Graduate School of Biomedical Sciences, 4Department of Genetics,University of Texas MD Anderson Cancer Center

Capítulos

  • 00:05Título
  • 00:44Preparation of Embryos
  • 03:32Preparation of Injection Solutions and Microinjection of Embryos
  • 08:22Fixation and Immunostaining of Embryos
  • 09:42Results: Examples of Embryos Targeted at the 8-cell Stage
  • 10:56Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.

Tags

MicroinjectionXenopus KidneyBiología del desarrolloTestis IsolationEgg CollectionFertilizationDe-jellingTemperature ControlMembrane-bound Red Fluorescent Protein MRNA

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