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Technique to Target Microinjection to the Developing Xenopus Kidney
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Technique to Target Microinjection to the Developing Xenopus Kidney
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11:29 min
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May 03, 2016
DOI:
1Department of Pediatrics, Pediatric Research Center,University of Texas McGovern Medical School, 2Program in Genes & Development,University of Texas Graduate School of Biomedical Sciences, 3Program in Cell & Regulatory Biology,University of Texas Graduate School of Biomedical Sciences, 4Department of Genetics,University of Texas MD Anderson Cancer Center
Summary
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Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.
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DeLay, B. D., Krneta-Stankic, V., Miller, R. K. Technique to Target Microinjection to the Developing Xenopus Kidney. J. Vis. Exp. (111), e53799, doi:10.3791/53799 (2016).
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