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HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
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JoVE Journal Nörobilim
HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice

HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice

DOI:
10.3791/53717-v

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07:30 min

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July 09, 2016

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, ,
1Psikiyatri Bölümü,Icahn School of Medicine at Mount Sinai, 2Department of Neurology,Icahn School of Medicine at Mount Sinai, 3Friedman Brain Institute,Icahn School of Medicine at Mount Sinai, 4Department of Physiology and Biophysics,Virginia Commonwealth University Medical School

Bölümler

  • 00:05Başlık
  • 00:56Stereotaxic Surgery to Introduce HSV Chimeric Constructs
  • 03:32Head Twitch Response Experiment
  • 05:04Data Review
  • 05:33Sonuçlar
  • 06:35Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This article describes how to inject viral vectors into the mouse frontal cortex to test behavioral assays that require GPCR heteromeric formation.

Etiketler

HSVTransgene ExpressionGPCR HeteromersMouse ModelBehavioral PhenotypesSerotonin 2aMetabotropic Glutamate Receptor 2Stereotactic SurgeryVirus-mediated Gene TransferAnesthesiaInjectionFrontal Cortex

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