'Dergi'
/
Gelişim Biyolojisi
/
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
JoVE Journal
Gelişim Biyolojisi
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Gelişim Biyolojisi
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

DOI:
10.3791/53654-v

•

11:25 min

•

February 22, 2016

•

, , , ,
1Developmental Biology Program,Sloan Kettering Institute

'Bölümler'

  • 00:05'Başlık'
  • 00:41Embryo Collection
  • 03:25Confocal Imaging
  • 04:47Image Analysis and Data Pre-processing
  • 08:44Results: Lineage Specification Analysis in Mouse Preimplantation Embryos
  • 10:30Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

This protocol presents a method to perform quantitative, single-cell in situ analysis of protein expression to study lineage specification in mouse preimplantation embryos. The procedures necessary for collection of blastocysts, whole-mount immunofluorescent detection of proteins, imaging of samples on a confocal microscope, and nuclear segmentation and image analysis are described.

'Etiketler'

Quantitative AnalysisProtein ExpressionLineage SpecificationMouse Preimplantation EmbryosConfocal MicroscopySingle-cell ResolutionSemi-automatedNuclear DensityCell Population HeterogeneityZona Pellucida RemovalImmunofluorescenceConfocal Imaging

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry

Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation

Read Article