/
/
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
10,701 Views
•
11:25 min
•
February 22, 2016
DOI:
Özet
Automatically generated
This protocol presents a method to perform quantitative, single-cell in situ analysis of protein expression to study lineage specification in mouse preimplantation embryos. The procedures necessary for collection of blastocysts, whole-mount immunofluorescent detection of proteins, imaging of samples on a confocal microscope, and nuclear segmentation and image analysis are described.
Read Article
Cite this Article
Saiz, N., Kang, M., Schrode, N., Lou, X., Hadjantonakis, A. Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos. J. Vis. Exp. (108), e53654, doi:10.3791/53654 (2016).
Copy