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Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
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JoVE Journal İmmünoloji ve Enfeksiyon
Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

11,988 Views

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06:20 min

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December 24, 2015

DOI:

10.3791/53265-v

DOI:
10.3791/53265-v

•

06:20 min
December 24, 2015

•

4 Views
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1Regenerative Medicine Research Group, Institute of Cellular & System Medicine,National Health Research Institutes (NHRI), 2National Institute of Cancer Research,National Health Research Institutes (NHRI), 3Institute of Microbiology & Immunology,National Defense Medical Center

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The immunomodulatory properties of human mesenchymal stem cells (MSC) appear increasingly relevant for clinical application. Using a co-culture system of MSCs and peripheral blood leukocytes pre-stained with the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE), we describe the in vitro assessment of MSC immunomodulation on effector leukocyte proliferation and specific subpopulations.

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Hsu, P., Liu, K., Chao, Y., Sytwu, H., Yen, B. L. Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs). J. Vis. Exp. (106), e53265, doi:10.3791/53265 (2015).

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