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Immunologie et infection
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Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
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Immunologie et infection
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Journal JoVE Immunologie et infection
Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

11,988 Views

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06:20 min

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December 24, 2015

DOI:

10.3791/53265-v

DOI:
10.3791/53265-v

•

06:20 min
December 24, 2015

•

4 Views
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1Regenerative Medicine Research Group, Institute of Cellular & System Medicine,National Health Research Institutes (NHRI), 2National Institute of Cancer Research,National Health Research Institutes (NHRI), 3Institute of Microbiology & Immunology,National Defense Medical Center

Summary

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The immunomodulatory properties of human mesenchymal stem cells (MSC) appear increasingly relevant for clinical application. Using a co-culture system of MSCs and peripheral blood leukocytes pre-stained with the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE), we describe the in vitro assessment of MSC immunomodulation on effector leukocyte proliferation and specific subpopulations.

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Cite this Article

Hsu, P., Liu, K., Chao, Y., Sytwu, H., Yen, B. L. Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs). J. Vis. Exp. (106), e53265, doi:10.3791/53265 (2015).

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